In the last ten years, forensic genetic analysis has progressed beyond traditional DNA profiling-based identification which links a suspect to crime scene evidence. The information provided by AIMs (Ancestry Informative Markers) can allow the differentiation of major population groups, offering information to investigators about a suspect in the absence of a database hit or eyewitness testimony. In the present study, a 300 AIM-SNPs panel has been developed to be analysed in one massively parallel sequencing (MPS) multiplex assay, by querying the main whole genome databases. The panel includes 286 biallelic and 14 tri-allelic loci which were selected with the aim of differentiating Africans, Americans, Europeans, Middle Eastern, North African, South Asian, East Asian, and Oceanian individuals. The differentiation power of the component ancestry SNPs was balanced to avoid bias in the estimation of co-ancestry proportions in admixed individuals. STRUCTURE genetic clustering software was used to assess the panel’s classification performance and assign ancestry to a total of 3,553 profiles, where a clear differentiation of the five main continental groups and the Eurasian sub-population groups has been achieved with a nested analysis approach.

In the last ten years, forensic genetic analysis has progressed beyond traditional DNA profiling-based identification which links a suspect to crime scene evidence. The information provided by AIMs (Ancestry Informative Markers) can allow the differentiation of major population groups, offering information to investigators about a suspect in the absence of a database hit or eyewitness testimony. In the present study, a 300 AIM-SNPs panel has been developed to be analysed in one massively parallel sequencing (MPS) multiplex assay, by querying the main whole genome databases. The panel includes 286 biallelic and 14 tri-allelic loci which were selected with the aim of differentiating Africans, Americans, Europeans, Middle Eastern, North African, South Asian, East Asian, and Oceanian individuals. The differentiation power of the component ancestry SNPs was balanced to avoid bias in the estimation of co-ancestry proportions in admixed individuals. STRUCTURE genetic clustering software was used to assess the panel’s classification performance and assign ancestry to a total of 3,553 profiles, where a clear differentiation of the five main continental groups and the Eurasian sub-population groups has been achieved with a nested analysis approach.

Ancestry analysis in Forensic Genetics: development of a new ancestry-informative SNPs panel

STAFFONI, GIORGIA
2021/2022

Abstract

In the last ten years, forensic genetic analysis has progressed beyond traditional DNA profiling-based identification which links a suspect to crime scene evidence. The information provided by AIMs (Ancestry Informative Markers) can allow the differentiation of major population groups, offering information to investigators about a suspect in the absence of a database hit or eyewitness testimony. In the present study, a 300 AIM-SNPs panel has been developed to be analysed in one massively parallel sequencing (MPS) multiplex assay, by querying the main whole genome databases. The panel includes 286 biallelic and 14 tri-allelic loci which were selected with the aim of differentiating Africans, Americans, Europeans, Middle Eastern, North African, South Asian, East Asian, and Oceanian individuals. The differentiation power of the component ancestry SNPs was balanced to avoid bias in the estimation of co-ancestry proportions in admixed individuals. STRUCTURE genetic clustering software was used to assess the panel’s classification performance and assign ancestry to a total of 3,553 profiles, where a clear differentiation of the five main continental groups and the Eurasian sub-population groups has been achieved with a nested analysis approach.
2021
Ancestry analysis in Forensic Genetics: development of a new ancestry-informative SNPs panel
In the last ten years, forensic genetic analysis has progressed beyond traditional DNA profiling-based identification which links a suspect to crime scene evidence. The information provided by AIMs (Ancestry Informative Markers) can allow the differentiation of major population groups, offering information to investigators about a suspect in the absence of a database hit or eyewitness testimony. In the present study, a 300 AIM-SNPs panel has been developed to be analysed in one massively parallel sequencing (MPS) multiplex assay, by querying the main whole genome databases. The panel includes 286 biallelic and 14 tri-allelic loci which were selected with the aim of differentiating Africans, Americans, Europeans, Middle Eastern, North African, South Asian, East Asian, and Oceanian individuals. The differentiation power of the component ancestry SNPs was balanced to avoid bias in the estimation of co-ancestry proportions in admixed individuals. STRUCTURE genetic clustering software was used to assess the panel’s classification performance and assign ancestry to a total of 3,553 profiles, where a clear differentiation of the five main continental groups and the Eurasian sub-population groups has been achieved with a nested analysis approach.
SNPs
Ancestry
Forensic Genetics
SNPs panel
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.12608/10241