Investigating the impact of permeability transition pore inhibitors on Glioblastoma multiforme (GBM) cell migration.

Glioblastoma multiforme (GBM) is the most widespread, highly heterogeneous, and aggressive brain tumor that is classified as a grade IV glioma. Understanding the mechanisms controlling GBM invasiveness poses a major clinical challenge in the field. Mitochondria play a central role in promoting cancer cell migration by supplying ATP. Recent studies have demonstrated that cyclosporin A (CsA), a pharmacological inhibitor of the permeability transition pore (PTP), a mitochondrial Ca2+-activated channel, affects GBM cell migration. However, the role of the PTP in the GBM context remains unexplored. My project aims to analyse PTP activity and the effects of its inhibitors on the migratory behaviour of multiple GBM cell lines, including U87, U251, and GE904. The results suggest that there is a link between PTP activity and the migration capacity of different GBM cell lines. Overall, GE904 and U251 cells were more responsive to inhibitor treatment than U87 cells, achieving the greatest suppression of migration.