Investigations into the role of the R2R3-MYB S4C2 clade on gene regulation in grapevine (Vitis vinifera L.)

Gene regulation is a turning point during plant growth, development and defense against biotic and abiotic stresses. Transcriptional factors (TFs) are the major switches during these processes. In plants, studying how these genes work is of relevant importance to improve crop quality and performance. In this work 3 classes of TFs in Vitis vinifera, NAC, WRKY and MYB were studied, deepening the role of 4 R2R3 MYB C2 repressors, MYBC2-L1, MYBC2-L2, MYBC2-L3 and MYB4b. A DNA affinity purification sequencing (DAP-seq) protocol was followed: gDNA was extracted from grapevine leaves and fragmented, while TFs were expressed in pIX:HALO tag. After TF-gDNA bounding and washing, DNA fragment were sequenced. The 4 TFs—related results were used to create gene co-expression analysis and infer the role of these genes. These results will provide knowledge to improve grapevine breeding and cultivation.