Detection and quantification of polyphenols in apple and orange varieties using HPLC and Spectrophotometric methods. Validation parameters of these methods were determined, including linearity, sensitivity, precision (intra-assay and intermediate), accuracy, LOD, and LOQ. For the validation process, groups of polyphenol standards were used, including phenolic acids (gallic, p-coumaric, caffeic, and chlorogenic acids), flavan-3-ols [(+)-catechin, and procyanidins B1 and B2], flavonols (quercetin and quercetin-3- rutinoside), and dihydrochalcones (phloretin and phloretin-2-glucoside). Obtained validation parameters were within acceptable ranges with high determination coefficients, reasonably low LODs and LOQs, and high slopes in the calibration curves for both methods, except for phloretin and phloretin-2-glucoside, for which there were low slopes in the calibration curves for the AlCl3 method. To evaluate differences in polyphenol content, the validated spectrophotometric methods were used to determine TP and TF content in apples and oranges according to polyphenol calibration curves. Polyphenol contents were different for both methods in all apple varieties and oranges.
Rilevazione e quantificazione dei polifenoli nelle varietà di mele e arance utilizzando Metodi HPLC e spettrofotometrici. Parametri di convalida di questi metodi sono stati determinati, tra cui linearità, sensibilità, precisione (intra-test e intermedio), precisione, LOD e LOQ. Per il processo di convalida, gruppi di sono stati utilizzati standard polifenolici, inclusi acidi fenolici (gallico, p-cumarico, caffeico e acidi clorogenici), flavan-3-oli [(+)-catechina e procianidine B1 e B2], flavonoli (quercetina e quercetina-3-rutinoside) e diidrocalconi (floretina e floretina-2-glucoside). I parametri di convalida ottenuti rientravano in intervalli accettabili con alti coefficienti di determinazione, LOD e LOQ ragionevolmente bassi e pendenze elevate nel curve di calibrazione per entrambi i metodi, ad eccezione di floretina e floretina-2-glucoside, per cui c'erano basse pendenze nelle curve di calibrazione per il metodo AlCl3. Per valutare le differenze nel contenuto di polifenoli, lo spettrofotometrico convalidato sono stati utilizzati metodi per determinare il contenuto di TP e TF in mele e arance secondo le curve di calibrazione dei polifenoli. Il contenuto di polifenoli era diverso per entrambi i metodi in tutte le varietà di mele e arance.
Caratterizzazione di polifenoli in mele e arance tramite spettrometria UV/Vis e tecnica HPLC
WAHAB, ABDUL
2021/2022
Abstract
Detection and quantification of polyphenols in apple and orange varieties using HPLC and Spectrophotometric methods. Validation parameters of these methods were determined, including linearity, sensitivity, precision (intra-assay and intermediate), accuracy, LOD, and LOQ. For the validation process, groups of polyphenol standards were used, including phenolic acids (gallic, p-coumaric, caffeic, and chlorogenic acids), flavan-3-ols [(+)-catechin, and procyanidins B1 and B2], flavonols (quercetin and quercetin-3- rutinoside), and dihydrochalcones (phloretin and phloretin-2-glucoside). Obtained validation parameters were within acceptable ranges with high determination coefficients, reasonably low LODs and LOQs, and high slopes in the calibration curves for both methods, except for phloretin and phloretin-2-glucoside, for which there were low slopes in the calibration curves for the AlCl3 method. To evaluate differences in polyphenol content, the validated spectrophotometric methods were used to determine TP and TF content in apples and oranges according to polyphenol calibration curves. Polyphenol contents were different for both methods in all apple varieties and oranges.File | Dimensione | Formato | |
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https://hdl.handle.net/20.500.12608/37438