Burkitt Lymphoma (BL) is a highly aggressive B-cell non Hodgkin lymphoma. It is the most frequent subtype of NHL in children and adolescents, accounting for 38% of patients from 0 to 14 years old. The current recommended approach of diagnosis and monitoring the disease’s spread comprises surgical removal and examination of the most accessible diseasecontaining tissue: consequentially, the excision is not representative only of the overall tumor. Liquid biopsy is becoming a very promising diagnostic method with several advantages over conventional invasive methods. Exosomes are among best candidates to be used as cancer biomarker from liquid biopsy, since their cargo mirrors the content of the cell of origin in both physiological and pathological intercellular communication. Recently, plasma exosomal microRNAs are assuming importance as valid circulating biomarkers for cancer diagnosis and monitoring. In this study, we followed the kinetics of specific microRNAs, found differentially enriched in plasmatic exosomes derived from pediatric BL patients compared to healthy donors. We identified miR-378a-3p as the best candidate as disease biomarker. In addition, we investigated the functional role of mir-378a-3p in BL cell lines
Burkitt Lymphoma (BL) is a highly aggressive B-cell non Hodgkin lymphoma. It is the most frequent subtype of NHL in children and adolescents, accounting for 38% of patients from 0 to 14 years old. The current recommended approach of diagnosis and monitoring the disease’s spread comprises surgical removal and examination of the most accessible diseasecontaining tissue: consequentially, the excision is not representative only of the overall tumor. Liquid biopsy is becoming a very promising diagnostic method with several advantages over conventional invasive methods. Exosomes are among best candidates to be used as cancer biomarker from liquid biopsy, since their cargo mirrors the content of the cell of origin in both physiological and pathological intercellular communication. Recently, plasma exosomal microRNAs are assuming importance as valid circulating biomarkers for cancer diagnosis and monitoring. In this study, we followed the kinetics of specific microRNAs, found differentially enriched in plasmatic exosomes derived from pediatric BL patients compared to healthy donors. We identified miR-378a-3p as the best candidate as disease biomarker. In addition, we investigated the functional role of mir-378a-3p in BL cell lines
Exosomal microRNAs as potential biomarkers in pediatric Burkitt Lymphoma
RIZZATO, DOMENICO
2021/2022
Abstract
Burkitt Lymphoma (BL) is a highly aggressive B-cell non Hodgkin lymphoma. It is the most frequent subtype of NHL in children and adolescents, accounting for 38% of patients from 0 to 14 years old. The current recommended approach of diagnosis and monitoring the disease’s spread comprises surgical removal and examination of the most accessible diseasecontaining tissue: consequentially, the excision is not representative only of the overall tumor. Liquid biopsy is becoming a very promising diagnostic method with several advantages over conventional invasive methods. Exosomes are among best candidates to be used as cancer biomarker from liquid biopsy, since their cargo mirrors the content of the cell of origin in both physiological and pathological intercellular communication. Recently, plasma exosomal microRNAs are assuming importance as valid circulating biomarkers for cancer diagnosis and monitoring. In this study, we followed the kinetics of specific microRNAs, found differentially enriched in plasmatic exosomes derived from pediatric BL patients compared to healthy donors. We identified miR-378a-3p as the best candidate as disease biomarker. In addition, we investigated the functional role of mir-378a-3p in BL cell lines| File | Dimensione | Formato | |
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https://hdl.handle.net/20.500.12608/42246