Regulation of C.elegans centrosome by deubiquitinase CYLD-1 and ubiquitinase MIB-1.

A centrosome is made up of two centrioles that are at right angles to one another and are surrounded by pericentriolar material. Centrosomes are the major microtubule organizing centers and they play an important role in cell division. Centrioles in C. elegans have a ninefold array of microtubular organizations formed by the sequential recruitment of centriolar proteins. Mutations in centrioles can lead to a broad range of diseases including developmental abnormalities, and cancer. A variety of factors can influence centriolar biogenesis, duplication, and stability. Understanding all the possible ways and aspects responsible for affecting the centriolar proteins could pave a path in the future to deal with abnormal centriole-related diseases. This study emphasizes in understanding the effects of depletion of a deubiquitinase CYLD-1, on SAS-7 a centriolar protein, and its effects on the first mitotic cell division in C. elegans. The second part of this study focuses on understanding the localization pattern of centriolar proteins SPD-2 and SAS-7 in C. elegans spermatozoa in fer-2, a mutant of MIB-1 ubiquitinase that functions in many systems as a counterpart to CYLD-1. The results from this study reveal that CYLD-1 depletion in C. elegans leads to an advancement of the first cell cycle with a phenotype of having an abnormal number of centrioles and resulting in the lethality of the worms. SPD-2 and SAS-7 centriolar proteins do not seem to change localization in MIB-1 spermatozoa.