The aim of this thesis project is to study and compare chemical and biogenic synthesis methodologies for obtaining silver (Ag) and gold (Au) nanoparticles. Currently these inorganic nanomaterials are widely studied for their chemical-physical and functional properties that make them the ideal candidates for the application in numerous fields of considerable technological interest. Nowadays Ag and Au nanoparticles are mainly synthetized through chemical synthesis (Turkevich synthesis) which results to be more expensive and energy-consuming than a biogenic approach that is considered a cheaper, safer and environmentally friendly method for obtaining inorganic nanomaterials. In this thesis work the biogenic agent used has been the black tea infusion, a beverage extremely popular throughout the world and easily available. Each type of synthesis was optimized by varying the various parameters to obtain high nanoparticles concentrations and to ensure the nanoparticles suspensions stability over time. The nanoparticles suspensions obtained were characterized with different analytical techniques; furthermore, considering their possible application in the biomedical field, their cytotoxicity in human colon cancer cells (HTC116) was investigated and correlated with the different synthesis approaches tested.
Il presente lavoro di Tesi ha come obiettivo lo studio e il confronto di metodologie di sintesi chimiche e biogeniche per l’ottenimento di nanoparticelle d’argento (Ag) e d’oro (Au). Attualmente, questi nanomateriali inorganici sono largamente studiati per le loro proprietà chimico-fisiche e funzionali, essendo dei candidati ideali per l’applicazione in numerosi campi di notevole interesse tecnologico. Oggigiorno, nanoparticelle di Ag e Au sono principalmente sintetizzate tramite via chimica (sintesi Turkevich), la quale risulta essere più costosa ed energeticamente dispendiosa rispetto a un approccio biogenico considerato un metodo economico, ecologico e sicuro per l’ottenimento di nanomateriali inorganici. In questo lavoro di Tesi, l’agente biogenico utilizzato è stato l’infuso di tè nero, bevanda estremamente diffusa in tutto il mondo e di facile reperibilità. Ogni tipologia di sintesi studiata è stata ottimizzata mediante la variazione sistematica di vari parametri con l’obiettivo di ottenere elevate concentrazioni di nanoparticelle e di assicurare la stabilità delle sospensioni nel tempo. Le sospensioni di nanoparticelle ottenute sono state caratterizzate con diverse tecniche analitiche; inoltre, considerando la loro possibile applicazione in ambito biomedico, è stata investigata la loro citotossicità in cellule umane di tumore al colon (HTC116), correlandola con i differenti approcci di sintesi testati.
Sintesi chimica e biogenica di nanoparticelle di argento e oro e studio della relativa citotossicità.
LEONCINI, MARTINA
2022/2023
Abstract
The aim of this thesis project is to study and compare chemical and biogenic synthesis methodologies for obtaining silver (Ag) and gold (Au) nanoparticles. Currently these inorganic nanomaterials are widely studied for their chemical-physical and functional properties that make them the ideal candidates for the application in numerous fields of considerable technological interest. Nowadays Ag and Au nanoparticles are mainly synthetized through chemical synthesis (Turkevich synthesis) which results to be more expensive and energy-consuming than a biogenic approach that is considered a cheaper, safer and environmentally friendly method for obtaining inorganic nanomaterials. In this thesis work the biogenic agent used has been the black tea infusion, a beverage extremely popular throughout the world and easily available. Each type of synthesis was optimized by varying the various parameters to obtain high nanoparticles concentrations and to ensure the nanoparticles suspensions stability over time. The nanoparticles suspensions obtained were characterized with different analytical techniques; furthermore, considering their possible application in the biomedical field, their cytotoxicity in human colon cancer cells (HTC116) was investigated and correlated with the different synthesis approaches tested.File | Dimensione | Formato | |
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https://hdl.handle.net/20.500.12608/45192