The goal of this study was to build a biosensor for copper that could detect its concentration in must. For this purpose, we transformed different strains of Saccharomyces Cerevisiae, including 330-BS, CESPLG05 and CENPK in order to produce a copper-dependent probe that is capable of emitting fluorescent signal in the presence of copper. This probe is consisting of a green fluorescent protein (GFP) reporter which has been used for easy detection and quantification of the copper levels. At the end we evaluated the performance of biosensor in each strain and compared the responses of each strain using different functional assays such as: microscopy, SDS-PAGE and Western-blot, and Fluorometer.
The goal of this study was to build a biosensor for copper that could detect its concentration in must. For this purpose, we transformed different strains of Saccharomyces Cerevisiae, including 330-BS, CESPLG05 and CENPK in order to produce a copper-dependent probe that is capable of emitting fluorescent signal in the presence of copper. This probe is consisting of a green fluorescent protein (GFP) reporter which has been used for easy detection and quantification of the copper levels. At the end we evaluated the performance of biosensor in each strain and compared the responses of each strain using different functional assays such as: microscopy, SDS-PAGE and Western-blot, and Fluorometer.
Construction of copper biosensor using Sacharomyces cerevisiae
ASADI, MARJAN
2022/2023
Abstract
The goal of this study was to build a biosensor for copper that could detect its concentration in must. For this purpose, we transformed different strains of Saccharomyces Cerevisiae, including 330-BS, CESPLG05 and CENPK in order to produce a copper-dependent probe that is capable of emitting fluorescent signal in the presence of copper. This probe is consisting of a green fluorescent protein (GFP) reporter which has been used for easy detection and quantification of the copper levels. At the end we evaluated the performance of biosensor in each strain and compared the responses of each strain using different functional assays such as: microscopy, SDS-PAGE and Western-blot, and Fluorometer.| File | Dimensione | Formato | |
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https://hdl.handle.net/20.500.12608/60136