Characterization of polymer-based nano-drug delivery systems to encapsulate proANP and non-coding RNAs (ncRNAs) for treating heart failure

Heart failure (HF) remains a significant global health concern, necessitating innovative therapeutic strategies. In this master's thesis, we focused on the development and characterization of polymer-based nano-drug delivery systems for encapsulating pro-atrial natriuretic peptide (proANP) and non-coding RNAs (ncRNAs) with the aim of advancing potential treatments for HF. In the initial phase, the polymer nanoparticles were meticulously characterized for morphology and stability using Transmission Electron Microscopy (TEM) and Dynamic Light Scattering (DLS). These analyses provided insights into the structural features and particle size distribution critical for optimizing the delivery system. To establish the safety of the developed nano-drug delivery system, cytotoxicity studies were conducted using the MTT assay on cardiomyocytes and macrophages. The results demonstrated the biocompatibility of the polymer nanoparticles, confirming their potential for therapeutic application without inducing adverse effects or activating immune responses. Subsequently, gene expression studies were undertaken to evaluate the impact of the nano-drug delivery system on specific genes associated with cardiac remodeling and inflammatory responses in immune cells. The findings shed light on the molecular interactions between the developed nanoparticles and cellular pathways relevant to HF, providing valuable insights into the potential therapeutic efficacy In conclusion, this master's thesis presents a comprehensive investigation into the characterization and safety assessment of polymer-based nano-drug delivery systems for proANP and ncRNAs encapsulation. The results highlight the potential of these nanostructures for targeted delivery in HF treatment, laying the groundwork for further advancements in cardiac therapeutics. This research contributes to the growing field of nanomedicine and holds promise for the development of novel strategies to address the complex pathophysiology of heart failure.