Type III polyketide synthase (PKSs) encodes for secondary metabolites with diverse biological activities, including antimicrobials. T3PKS genes are regularly reported in genomes of fungi, whereby exploring the diversity of these enzymes would be the starting point to exploit T3PKS to produce novel bioactive compounds. T3PKS genes are spread among non-lichen forming fungi, but the presence and diversity of T3PKs in lichenized fungi is still largely unknown. My study aims to investigate the diversity of this class of compounds in lichen-forming fungi, sheding light on the possible evolutionary origins and relatedness to those present in non-lichenized fungi. Starting from a dataset of 411 genomes retrieved from NCBI, belonging both to Ascomycota and Basidiomycota, we extracted the T3PKSs sequences of different life-style fungi. The alignement of the sequences produced a 1000 bootstrap maximum likelihood tree that was further analyzed, and compared to a phylogenetic tree, to move forward a comprehensive understanding of the evolution of these gene clusters in lichenized fungi.
Type III polyketide synthase (PKSs) encodes for secondary metabolites with diverse biological activities, including antimicrobials. T3PKS genes are regularly reported in genomes of fungi, whereby exploring the diversity of these enzymes would be the starting point to exploit T3PKS to produce novel bioactive compounds. T3PKS genes are spread among non-lichen forming fungi, but the presence and diversity of T3PKs in lichenized fungi is still largely unknown. My study aims to investigate the diversity of this class of compounds in lichen-forming fungi, sheding light on the possible evolutionary origins and relatedness to those present in non-lichenized fungi. Starting from a dataset of 411 genomes retrieved from NCBI, belonging both to Ascomycota and Basidiomycota, we extracted the T3PKSs sequences of different life-style fungi. The alignement of the sequences produced a 1000 bootstrap maximum likelihood tree that was further analyzed, and compared to a phylogenetic tree, to move forward a comprehensive understanding of the evolution of these gene clusters in lichenized fungi.
Comparative genomics of fungal type III polyketide synthase, with focus on lichen-forming fungi
MAGLIONE, LAURA
2023/2024
Abstract
Type III polyketide synthase (PKSs) encodes for secondary metabolites with diverse biological activities, including antimicrobials. T3PKS genes are regularly reported in genomes of fungi, whereby exploring the diversity of these enzymes would be the starting point to exploit T3PKS to produce novel bioactive compounds. T3PKS genes are spread among non-lichen forming fungi, but the presence and diversity of T3PKs in lichenized fungi is still largely unknown. My study aims to investigate the diversity of this class of compounds in lichen-forming fungi, sheding light on the possible evolutionary origins and relatedness to those present in non-lichenized fungi. Starting from a dataset of 411 genomes retrieved from NCBI, belonging both to Ascomycota and Basidiomycota, we extracted the T3PKSs sequences of different life-style fungi. The alignement of the sequences produced a 1000 bootstrap maximum likelihood tree that was further analyzed, and compared to a phylogenetic tree, to move forward a comprehensive understanding of the evolution of these gene clusters in lichenized fungi.File | Dimensione | Formato | |
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https://hdl.handle.net/20.500.12608/78231