Hydrogel-integrated microfluidics for spatial patterning of neural organoids through morphogen gradients

Achieving precise spatial patterning of neural tissues in vitro relies on the controlled delivery of morphogens within a well-defined microenvironment. This thesis explores a hydrogel-integrated microfluidic device capable of generating stable and tunable gradients of signaling molecules involved in dorsoventral neural tube patterning. The goal is to assess whether neural organoids respond to these gradients with localized differentiation, offering a more accurate model of early neurodevelopmental processes.