Dinamica del microbioma e cura della pelle a base vegetale: Inibire i microrganismi patogeni nella pelle danneggiata

The skin microbiome plays a vital role in maintaining cutaneous health, with disruptions contributing to various dermatological conditions. Staphylococcus aureus, a key pathogenic bacterium, is frequently associated with skin inflammation and barrier dysfunction. This thesis investigates the antimicrobial potential of Chlorella sorokiniana, a thermophilic green microalga, specifically against S. aureus. Bioactive compounds were extracted using acetone and DMSO, and characterized via LC-MS, QTOF, UV-Vis spectrophotometry, and CHNS analysis. Identified metabolites—such as astaxanthin, lutein, β-sitosterol, and kushenol derivatives—demonstrated significant antimicrobial activity in vitro. Using MIC and disk diffusion assays, C. sorokiniana extracts inhibited S. aureus growth, highlighting their potential as natural, microbiome-supportive agents in skincare formulations targeting pathogenic colonization and inflammation.