STUDY OF THE EFFECT OF PHENOLIC COMPOUNDS AND/OR MECHANICAL LOADING ON OSTEOBLAST-LIKE MG-63 CELLS.

Bone remodeling functions as an ongoing process throughout life to preserve skeletal strength and handle physical requirements and fix tiny bone injuries. Bone remodeling depends on equal activities between different bone cells because osteoblasts specifically create bone tissue. Bone remodeling processes receive their most significant influence from mechanical forces among all factors that affect this process. Bone tissue shows extreme sensitivity to mechanical changes because cells possess the ability to detect physical stimuli including tension and compression. The cells initiate particular signaling pathways which manage gene expression together with protein synthesis and cell differentiation processes. The regulation of osteoblast function depends on multiple factors beyond mechanical signals: on environmental factors including diet as well as oxidative stress and bioactive compounds. Recent scientific interest focuses on natural and synthetic antioxidant molecules because they protect cells from oxidative damage while influencing cellular responses. The natural polyphenol hydroxy-tyrosol (HT) found in olive oil demonstrates promising biological effects alongside the synthetic antioxidant compound FRAKO503 (FR). This research investigates how MG-63 osteoblast-like cells respond to mechanical stimulation during laboratory experiments and evaluates how antioxidant compounds HT and FR impact this cellular response. The research used a strain-mimicking system for mechanical loading and antioxidant molecule treatments to study their protective and combined effects. The research investigates how physical stimulation interacts with antioxidant treatment to understand complex bone cell responses. Research into these interactions could lead to the development of new approaches to enhance bone regeneration and treat osteoporosis and other bone related diseases that result from oxidative stress and insufficient mechanical activity.