Vaginal cytology in bitches is a useful and handly tool in everyday clinical practice, allowing us to determine the stage of the estrous cyle; this technique can be useful also for the investigation of some gynecological pathologies. Being able to identify the day of the ovulation is essential for the correct management of artificial insemination procedures and for a successful mating. The information obtained from vaginal cytology is usually associated with the measurement of serum progesterone concentration, which makes the evaluation of the optimal mating time even more accurate in determining the phase of the estrous cycle. In this project we obtained a detailed photographic collection for teaching purpose and use. The future aim would be to use these pictures for the realization of a photographic atlas about cells componing the normal vaginal cytology. The pictures were obtained with a Nikon video camera associated with a microscope and the pictures were optimized and edited with NIS Elements, a software used for the acquisition of medical images. Complementary to this work, we added a differential cell count at the picture collection. For this purpose, we examined 3 slides for each phase of the estrous cylce, and for each of them we take 2 scans in different points of the slide. Subsequently, we performed a count of 200 cells per scan and the various cell was classified according to the different cell type that we can find in a smear; in this way we have obtained a representation of the variation in the quantification of a cell type in relation to the various phases of the reproductive cycle.
La citologia vaginale nella specie canina è uno strumento efficace e comodo nella pratica ambulatoriale di tutti i giorni per la determinazione dello stadio del ciclo estrale e per indagare eventuali patologie ginecologiche. Riuscire ad identificare con precisione il giorno dell’ovulazione è fondamentale per una corretta gestione delle procedure di inseminazione artificiale e per far sì che l’accoppiamento in generale possa andare a buon fine. Le informazioni ricavate dalla colpocitologia sono solitamente affiancate alla misurazione della concentrazione di progesterone sierico che permette di rendere ancora più attendibile la determinazione della fase del ciclo estrale. Questo studio nasce dall’idea di raccogliere materiale fotografico a fini didattici riguardante tutte le varie cellule che si possono trovare all’interno di uno striscio vaginale, con l’obiettivo futuro di poter sfruttare questo materiale per la realizzazione di un atlante fotografico. Le foto sono state ottenute attraverso una videocamera Nikon associata ad un microscopio e sono poi state ottimizzate ed elaborate grazie al software NIS Elements, usato per l’acquisizione delle immagini mediche. Alla raccolta fotografica è stato poi aggiunto uno studio riguardante la conta cellulare differenziale dei preparati citologici; sono stati presi in esame 3 vetrini per ogni fase del ciclo, e per ognuno di essi sono state acquisite 2 scansioni in punti diversi del vetrino. Successivamente è stata effettuata la conta di 200 cellule per scansione, classificandole in base al tipo cellulare; questo ha permesso di avere una rappresentazione della variabilità della quantità di un determinato tipo cellulare in relazione alle varie fasi del ciclo.
Citologia vaginale della cagna: studio fotografico e conta cellulare differenziale nelle varie fasi del ciclo
ZILIO, SOFIA
2022/2023
Abstract
Vaginal cytology in bitches is a useful and handly tool in everyday clinical practice, allowing us to determine the stage of the estrous cyle; this technique can be useful also for the investigation of some gynecological pathologies. Being able to identify the day of the ovulation is essential for the correct management of artificial insemination procedures and for a successful mating. The information obtained from vaginal cytology is usually associated with the measurement of serum progesterone concentration, which makes the evaluation of the optimal mating time even more accurate in determining the phase of the estrous cycle. In this project we obtained a detailed photographic collection for teaching purpose and use. The future aim would be to use these pictures for the realization of a photographic atlas about cells componing the normal vaginal cytology. The pictures were obtained with a Nikon video camera associated with a microscope and the pictures were optimized and edited with NIS Elements, a software used for the acquisition of medical images. Complementary to this work, we added a differential cell count at the picture collection. For this purpose, we examined 3 slides for each phase of the estrous cylce, and for each of them we take 2 scans in different points of the slide. Subsequently, we performed a count of 200 cells per scan and the various cell was classified according to the different cell type that we can find in a smear; in this way we have obtained a representation of the variation in the quantification of a cell type in relation to the various phases of the reproductive cycle.| File | Dimensione | Formato | |
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ZILIO_SOFIA.pdf
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https://hdl.handle.net/20.500.12608/56130