Isolation of compounds from Taraxacum officinale roots by chromatographic methods.

The research explores the phytochemical composition of extracts derived from the roots of Taraxacum officinale, commonly known as dandelion. Pharmacological investigations have previously indicated the diverse medicinal properties of dandelion due to the presence of various bioactive compounds in its extracts. These properties include anti-inflammatory, antinociceptive, antioxidant, and anticancer activities. This study aimed to isolate and characterize the phytochemical constituents of Taraxacum officinale roots through techniques such as column chromatography, prep-TLC, and prep-HPLC. Through spectroscopic analyses including 1H-NMR and 13C-NMR, mass spectrometry (GC-MS) compounds such as β-sitosterol, stigmasterol, taraxasterol acetate, lupeol, α-amyrin, and β-amyrin were identified.