Optimising microalgal biodiesel: enhancing lipid extraction and transesterification efficiency

The global quest for sustainable energy solutions has intensified as the adverse effects of fossil fuel consumption become increasingly evident. The increasing energy demand makes it urgent to seek alternative biofuel production derived from biological sources that allow to reduce dependence on fossil fuels. Among various biomass sources, microalgae have gained significant attention due to their high growth rates, minimal water and nutrient requirements, and ability to fix atmospheric CO2 efficiently. This thesis investigates the potential of microalgae as a promising biodiesel feedstock, with a primary focus on the production phase. It analyses lipid extraction techniques and bioconversion through advanced combinatorial approaches in transesterification processes, aiming to optimise reaction conditions for maximum yield. Furthermore, it explores the potential of genetic and biochemical engineering to boost productivity, thus optimising the biofuel production pathway.