RNAi-mediated gene silencing in Halyomorpha halys: Effects on Life History Parameters and Gene Expression

The Brown Marmorated Stink Bug (BMSB), Halyomorpha halys (Stål)1855; Heteropteran: Pentatomidae), is a phytophagous bug indigenous to Asia that has become a rapidly expanding invasive species in Europe with a vast host range and high damage potential. Current management tactics primarily rely on mass trapping and synthetic pesticides, highlighting the need for alternative measures. RNA interference (RNAi) is a gene silencing technique in which the double-stranded RNA (dsRNA) inhibits the gene expression by degrading the target mRNA, which could be a promising sustainable tool. This study aims to evaluate the effectiveness of 8 dsRNA (Rpn7, elF3a, PREB, Rps3a, srp54K, hsc70, shi, ROP), which were selected from previous genome-wide screens identified as essential genes involved in fundamental cellular processes like gene expression and protein homeostasis. The dsRNA is injected in adult H.halys, focusing on the effects on various life history traits like mortality, fecundity, feeding ability, and the survival and development time of the F1 generation. Quantitative RT-PCR is carried out to determine the knockdown efficiency of the target genes. Results from this study could provide insights into the potential of RNAi for managing H.halys populations and reducing reliance on chemical pesticides.