ABSTRACT Polyalthia longifolia is a traditional medicinal plant of the Annonaceae family. It is used in Asia and is cultivated in India, Pakistan and Sri Lanka. It is often used as an antipyretic agent. Some pharmacological studies have described antiulcer activity, hypotensive effects and also hypoglycemic action. The aim of this experimental thesis project was to study six extracts of Polyalthia longifolia in vitro, with a view to evaluating their potential use in the prevention of diabetes mellitus and the damage caused by this disease. Aqueous, methanol and ethanolic extracts obtained from leaves and stems of Polyalthia longifolia were studied. As a first approach, the potential hypoglycemic activity was assessed by performing the α-glucosidase inhibition test measuring the reduction of enzyme activity from the samples under consideration. The BSA (Bovin Serum Albumin) test was used to evaluate the antiglycative activity, while the TPC (Total Phenol Content) and TFC (Total Flavonoid Content) tests were carried out with the aim of determining the content of phenolic and flavonoid compounds, Determining the antioxidant potential of Polyalthia longifolia extracts. A second phase of the research was devoted to the study of the cytotoxicity of extracts by means of the MTT test useful for determining the antiproliferative effects. This evaluation was conducted on intestinal cell cultures called HT-29 (Human colorectal adenocarcinoma cell line).
ABSTRACT Polyalthia longifolia è una pianta medicinale di uso tradizionale della famiglia delle Annonaceae. Si utilizza in Asia ed è coltivata in India, Pakistan e Sri Lanka. Trova spesso impiego come agente antipiretico. Alcuni studi farmacologici hanno descritto attività antiulcera, effetti ipotensivi e anche azione ipoglicemizzante. Il presente progetto di tesi sperimentale si è posto come obiettivo lo studio in vitro di sei estratti di Polyalthia longifolia allo scopo di valutarne il potenziale impiego nella prevenzione del diabete mellito e dei danni causati da tale patologia. Sono stati studiati estratti acquosi, metanolici ed etanolici ottenuti da foglie e fusti di Polyalthia longifolia. Come primo approccio è stata valutata la potenziale attività ipoglicemizzante tramite lo svolgimento del saggio d’inibizione dell’α-glucosidasi misurando la riduzione dell’attività enzimatica da parte dei campioni in esame. Il saggio BSA (Bovin Serum Albumin) è stato impiegato per valutare l’attività antiglicativa, mentre i saggi TPC (Total Phenol Content) e TFC (Total Flavonoid Content) sono stati svolti con lo scopo di determinare il contenuto dei composti fenolici e flavonoidici, determinando il potenziale antiossidante degli estratti di Polyalthia longifolia. Una seconda fase della ricerca è stata dedicata allo studio della citotossicità degli estratti tramite il saggio MTT utile per determinare gli effetti antiproliferativi. Tale valutazione è stata condotta su colture cellulari intestinali denominate HT-29 (Human colorectal adenocarcinoma cell line).
Studio delle proprietà ipoglicemizzanti e antiglicanti di estratti vegetali ottenuti da foglie e fusti di Polyalthia longifolia.
TOMASI, LAURA
2023/2024
Abstract
ABSTRACT Polyalthia longifolia is a traditional medicinal plant of the Annonaceae family. It is used in Asia and is cultivated in India, Pakistan and Sri Lanka. It is often used as an antipyretic agent. Some pharmacological studies have described antiulcer activity, hypotensive effects and also hypoglycemic action. The aim of this experimental thesis project was to study six extracts of Polyalthia longifolia in vitro, with a view to evaluating their potential use in the prevention of diabetes mellitus and the damage caused by this disease. Aqueous, methanol and ethanolic extracts obtained from leaves and stems of Polyalthia longifolia were studied. As a first approach, the potential hypoglycemic activity was assessed by performing the α-glucosidase inhibition test measuring the reduction of enzyme activity from the samples under consideration. The BSA (Bovin Serum Albumin) test was used to evaluate the antiglycative activity, while the TPC (Total Phenol Content) and TFC (Total Flavonoid Content) tests were carried out with the aim of determining the content of phenolic and flavonoid compounds, Determining the antioxidant potential of Polyalthia longifolia extracts. A second phase of the research was devoted to the study of the cytotoxicity of extracts by means of the MTT test useful for determining the antiproliferative effects. This evaluation was conducted on intestinal cell cultures called HT-29 (Human colorectal adenocarcinoma cell line).| File | Dimensione | Formato | |
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https://hdl.handle.net/20.500.12608/73586