Genomic characterization and diversity assessment through NGS approach of Trifolium populations

This study aims to perform a genomic characterisation and genetic diversity assessment of various Trifolium populations using a next-generation sequencing (NGS) approach, specifically through the ddRADseq technique. Samples representing Trifolium incarnatum and Trifolium alexandrinum were analysed to uncover both intraspecific and interspecific variation. High-throughput sequencing data were used to evaluate population structure, genetic distances, and phylogenetic relationships among accessions. The results were interpreted in the context of the DUS (Distinctness, Uniformity, Stability) criteria, which are essential for cultivar identification and registration. This research contributes valuable insights into the genetic makeup of Trifolium species and supports future breeding programmes and molecular-based cultivar distinction in forage legume improvement strategies.