The main purpose of this thesis is the design and development of an in vitro acoustic stimulation system through the structuring of a soundproof place and a controlled cell stimulation to obtain preliminary results. This thesis stems from the analysis made in the literature that shows results obtained from various studies over the years that do not allow a comparison between them because of different biological material used in addition to different sound stimuli on a frequency and intensity basis. For the realization of the acoustic stimulation system, was used a plexiglass cell incubator, subsequently soundproofed through the use of internal and external sound absorbing panels; a measuring microphone and two 45watt speakers to reproduce the acoustic stimulus sent by the REW software. A sound meter and measuring microphone were used to test the effectiveness of the system, and high-intensity stimuli were sent via the REW software to test the strength of the speakers. Subsequently, the viability of the biological material inside the built incubator was verified; the biological models, without production of stimuli, were placed inside the incubator and viewed after 1, 2 and 3 hours. Once it was accurate that the vitality remained unchanged, the sound stimulation phase began to verify the induction of sound damage to the cellular material. In practice they were subjected to stimuli of 1000-2000hz, 2000-4000Hz and 4000-8000 Hz at 100db for 20 minutes. In conclusion, the preliminary data we have obtained demonstrate a lower vitality of the cellular material tested with stimuli 2000-4000Hz.
Lo scopo principale di questa tesi è il disegno e la messa a punto un sistema di stimolazione acustica in vitro attraverso la strutturazione di un luogo insonorizzato e di una stimolazione cellullare controllata per ottenere risultati preliminari. Questa tesi nasce dall’analisi fatta in letteratura che mostra risultati ottenuti da vari studi negli anni che non permettono un confronto tra loro a causa di diverso materiale biologico utilizzato oltre che a differenti stimoli sonori su base di frequenza ed intensità. Per la realizzazione del sistema di stimolazione acustica è stata utilizzata un’incubatrice cellulare di plexiglass, successivamente insonorizzata attraverso l’utilizzo di pannelli fono assorbenti interni ed esterni; un microfono da misurazione e due casse da 45watt per riprodurre lo stimolo acustico inviato dal software REW. Per verificare l’efficacia del sistema sono stati utilizzati un fonometro e il microfono da misurazione per testare l’effettiva insonorizzazione, e tramite il software REW son o stati inviati stimoli ad alta intensità per testare la resistenza delle casse. Successivamente è stata verificata la vitalità del materiale biologico all’interno dell’incubatrice costruita; sono quindi stati posizionati all’interno dell’incubatrice i modelli biologici, senza produzione di stimoli, e visionati dopo 1, 2 e 3 ore. Una volta accurato che la vitalità restava invariata è iniziata la fase di stimolazione sonora per verificare l’induzione di danno sonoro al materiale cellulare. In pratica sono stati sottoposti a stimoli pari a 1000-2000Hz, 2000-4000Hz e 4000-8000 Hz a 100db per 20 minuti. In conclusione, i dati preliminari che abbiamo ottenuto dimostrano una minor vitalità del materiale cellulare testate con stimoli 2000-4000Hz.
Disegno e messa a punto di un sistema di stimolazione acustica in vitro: risultati preliminari.
BIANCO, ALESSIA
2022/2023
Abstract
The main purpose of this thesis is the design and development of an in vitro acoustic stimulation system through the structuring of a soundproof place and a controlled cell stimulation to obtain preliminary results. This thesis stems from the analysis made in the literature that shows results obtained from various studies over the years that do not allow a comparison between them because of different biological material used in addition to different sound stimuli on a frequency and intensity basis. For the realization of the acoustic stimulation system, was used a plexiglass cell incubator, subsequently soundproofed through the use of internal and external sound absorbing panels; a measuring microphone and two 45watt speakers to reproduce the acoustic stimulus sent by the REW software. A sound meter and measuring microphone were used to test the effectiveness of the system, and high-intensity stimuli were sent via the REW software to test the strength of the speakers. Subsequently, the viability of the biological material inside the built incubator was verified; the biological models, without production of stimuli, were placed inside the incubator and viewed after 1, 2 and 3 hours. Once it was accurate that the vitality remained unchanged, the sound stimulation phase began to verify the induction of sound damage to the cellular material. In practice they were subjected to stimuli of 1000-2000hz, 2000-4000Hz and 4000-8000 Hz at 100db for 20 minutes. In conclusion, the preliminary data we have obtained demonstrate a lower vitality of the cellular material tested with stimuli 2000-4000Hz.File | Dimensione | Formato | |
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https://hdl.handle.net/20.500.12608/57497