Cancer is a serious and widespread disease, representing the main cause of death in the world. In recent years, research has been looking for alternative therapies that can replace or combine with traditional ones to increase their effectiveness and minimize their side effects. Nanomedicine, and in particular magnetic nanoparticles (MNPs), are a possible alternative, since they can act as targeted drug nanocarriers (chemotherapy) that can be specifically directed to the tumor; they also experience localized heating that causes cell death (hyperthermia) when exposed to an alternating magnetic field (AMF) or can cause mechanical damages to cells when exposed to a rotating magnetic field (RMF). Hyperthermia, in addition to being produced by exposing MNPs to an alternating magnetic field, is also produced by the radiation of a laser beam in the near-infrared region (NIR), at a wavelength at which tissues become partially transparent. In this work were used Biomimetic magnetic nanoparticles (BMNPs) mediated by magnetosome protein MamC that have a wide range of biomedical applications due to their unique characteristics, such as good magnetic properties (they are superparamagnetic and possess a large magnetic moment per particle), biocompatibility and specific surface properties. This makes them good candidate to act as magnetic hyperthermia agents, photothermal agents and to be exposed to a RMF; all these functions are studied in this work. Initially the internalization of BMNPs was studied in two cell lines, one tumoral (MCF7) and one non-tumor (MCF10A), to verify that the internalization was greater in the tumoral one. Subsequently the effects of magnetic hyperthermia, photothermia, and rotating magnetic field on the viability of tumor and non-tumor cell lines were carried out to verify the effectiveness of these techniques and to determine the best in treating tumors, in this case Breast cancer. The results of the study show that magnetic hyperthermia is more effective than photothermia and rotating magnetic field in inducing cell death in tumor cells, while none of the three techniques affects the cell viability of non-tumor cells demonstrating their high possibility of use in the field of tumor nanomedicine.

Cancer is a serious and widespread disease, representing the main cause of death in the world. In recent years, research has been looking for alternative therapies that can replace or combine with traditional ones to increase their effectiveness and minimize their side effects. Nanomedicine, and in particular magnetic nanoparticles (MNPs), are a possible alternative, since they can act as targeted drug nanocarriers (chemotherapy) that can be specifically directed to the tumor; they also experience localized heating that causes cell death (hyperthermia) when exposed to an alternating magnetic field (AMF) or can cause mechanical damages to cells when exposed to a rotating magnetic field (RMF). Hyperthermia, in addition to being produced by exposing MNPs to an alternating magnetic field, is also produced by the radiation of a laser beam in the near-infrared region (NIR), at a wavelength at which tissues become partially transparent. In this work were used Biomimetic magnetic nanoparticles (BMNPs) mediated by magnetosome protein MamC that have a wide range of biomedical applications due to their unique characteristics, such as good magnetic properties (they are superparamagnetic and possess a large magnetic moment per particle), biocompatibility and specific surface properties. This makes them good candidate to act as magnetic hyperthermia agents, photothermal agents and to be exposed to a RMF; all these functions are studied in this work. Initially the internalization of BMNPs was studied in two cell lines, one tumoral (MCF7) and one non-tumor (MCF10A), to verify that the internalization was greater in the tumoral one. Subsequently the effects of magnetic hyperthermia, photothermia, and rotating magnetic field on the viability of tumor and non-tumor cell lines were carried out to verify the effectiveness of these techniques and to determine the best in treating tumors, in this case Breast cancer. The results of the study show that magnetic hyperthermia is more effective than photothermia and rotating magnetic field in inducing cell death in tumor cells, while none of the three techniques affects the cell viability of non-tumor cells demonstrating their high possibility of use in the field of tumor nanomedicine.

EVALUATING THE EFFECTS OF MAGNETIC HYPERTHERMIA, PHOTOTHERMIA, AND ROTATING MAGNETIC FIELD ON THE VIABILITY OF TUMOR VS. NON-TUMOR CELL LINES

BUCCETTI, LARA
2023/2024

Abstract

Cancer is a serious and widespread disease, representing the main cause of death in the world. In recent years, research has been looking for alternative therapies that can replace or combine with traditional ones to increase their effectiveness and minimize their side effects. Nanomedicine, and in particular magnetic nanoparticles (MNPs), are a possible alternative, since they can act as targeted drug nanocarriers (chemotherapy) that can be specifically directed to the tumor; they also experience localized heating that causes cell death (hyperthermia) when exposed to an alternating magnetic field (AMF) or can cause mechanical damages to cells when exposed to a rotating magnetic field (RMF). Hyperthermia, in addition to being produced by exposing MNPs to an alternating magnetic field, is also produced by the radiation of a laser beam in the near-infrared region (NIR), at a wavelength at which tissues become partially transparent. In this work were used Biomimetic magnetic nanoparticles (BMNPs) mediated by magnetosome protein MamC that have a wide range of biomedical applications due to their unique characteristics, such as good magnetic properties (they are superparamagnetic and possess a large magnetic moment per particle), biocompatibility and specific surface properties. This makes them good candidate to act as magnetic hyperthermia agents, photothermal agents and to be exposed to a RMF; all these functions are studied in this work. Initially the internalization of BMNPs was studied in two cell lines, one tumoral (MCF7) and one non-tumor (MCF10A), to verify that the internalization was greater in the tumoral one. Subsequently the effects of magnetic hyperthermia, photothermia, and rotating magnetic field on the viability of tumor and non-tumor cell lines were carried out to verify the effectiveness of these techniques and to determine the best in treating tumors, in this case Breast cancer. The results of the study show that magnetic hyperthermia is more effective than photothermia and rotating magnetic field in inducing cell death in tumor cells, while none of the three techniques affects the cell viability of non-tumor cells demonstrating their high possibility of use in the field of tumor nanomedicine.
2023
EVALUATING THE EFFECTS OF MAGNETIC HYPERTHERMIA, PHOTOTHERMIA, AND ROTATING MAGNETIC FIELD ON THE VIABILITY OF TUMOR VS. NON-TUMOR CELL LINES
Cancer is a serious and widespread disease, representing the main cause of death in the world. In recent years, research has been looking for alternative therapies that can replace or combine with traditional ones to increase their effectiveness and minimize their side effects. Nanomedicine, and in particular magnetic nanoparticles (MNPs), are a possible alternative, since they can act as targeted drug nanocarriers (chemotherapy) that can be specifically directed to the tumor; they also experience localized heating that causes cell death (hyperthermia) when exposed to an alternating magnetic field (AMF) or can cause mechanical damages to cells when exposed to a rotating magnetic field (RMF). Hyperthermia, in addition to being produced by exposing MNPs to an alternating magnetic field, is also produced by the radiation of a laser beam in the near-infrared region (NIR), at a wavelength at which tissues become partially transparent. In this work were used Biomimetic magnetic nanoparticles (BMNPs) mediated by magnetosome protein MamC that have a wide range of biomedical applications due to their unique characteristics, such as good magnetic properties (they are superparamagnetic and possess a large magnetic moment per particle), biocompatibility and specific surface properties. This makes them good candidate to act as magnetic hyperthermia agents, photothermal agents and to be exposed to a RMF; all these functions are studied in this work. Initially the internalization of BMNPs was studied in two cell lines, one tumoral (MCF7) and one non-tumor (MCF10A), to verify that the internalization was greater in the tumoral one. Subsequently the effects of magnetic hyperthermia, photothermia, and rotating magnetic field on the viability of tumor and non-tumor cell lines were carried out to verify the effectiveness of these techniques and to determine the best in treating tumors, in this case Breast cancer. The results of the study show that magnetic hyperthermia is more effective than photothermia and rotating magnetic field in inducing cell death in tumor cells, while none of the three techniques affects the cell viability of non-tumor cells demonstrating their high possibility of use in the field of tumor nanomedicine.
BMNP
Hyperthermia
Photothermia
RMF
File in questo prodotto:
File Dimensione Formato  
Buccetti_Lara.pdf

accesso aperto

Dimensione 15.6 MB
Formato Adobe PDF
15.6 MB Adobe PDF Visualizza/Apri

The text of this website © Università degli studi di Padova. Full Text are published under a non-exclusive license. Metadata are under a CC0 License

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.12608/80834